Human Gamma-Thrombin: Lack of Correlation Between a Platelet Functional Response and Glycoprotein V Hydrolysis

Martine Jandrot-Perrus; Marie-Claude Guillin; Alan T Nurden

doi:10.1055/s-0038-1646015

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1987; 58(03): 915-920
DOI: 10.1055/s-0038-1646015

Original Article

Schattauer GmbH Stuttgart

Human Gamma-Thrombin: Lack of Correlation Between a Platelet Functional Response and Glycoprotein V Hydrolysis

Martine Jandrot-Perrus

The Unité 150 INSERM and Unité Associée 334 CNRS, Hôpital Lariboisiére, Paris, and Laboratoire de Recherche sur I’Hémostase et la Thrombose, Faculté Xavier Bichat, Paris, France

,

Marie-Claude Guillin

The Unité 150 INSERM and Unité Associée 334 CNRS, Hôpital Lariboisiére, Paris, and Laboratoire de Recherche sur I’Hémostase et la Thrombose, Faculté Xavier Bichat, Paris, France

,

Alan T Nurden

The Unité 150 INSERM and Unité Associée 334 CNRS, Hôpital Lariboisiére, Paris, and Laboratoire de Recherche sur I’Hémostase et la Thrombose, Faculté Xavier Bichat, Paris, France

› Author Affiliations

Abstract

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Summary

The ability of purified human gamma-thrombin to stimulate platelet function was related to its capacity to degrade GP V. Compared to alpha-thrombin, much greater amounts of gamma- thrombin were required to induce platelet aggregation; and this also applied to secretion from dense bodies, alpha-granules and lysosomal granules. Platelet stimulation by gamma-thrombin was additionally characterized by the presence of a lag-phase. Platelets with ³H-labelled surface glycoproteins showed the same functional response to both alpha- and gamma-thrombin as unlabelled platelets. But while threshold levels of alpha-thrombin induced little GP V hydrolysis confirming McGowan et al. (1), amounts of gamma-thrombin which induced substantial degradation (e. g. 8.3 nM degraded 36% of platelet GP V in 3 min) were unable to sustain either platelet aggregation or secretion. These results suggest that protein-binding regions remote from the catalytic site of alpha-thrombin are more important for platelet activation than GP V hydrolysis. They also provide further support to the argument that GP V hydrolysis may not be the essential trigger of platelet activation by thrombin.

Keywords

Platelet aggregation - Platelet secretion - GP V hydrolysis - Human alpha-thrombin - Human gamma-thrombin

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References

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